FastQCFastQC Report
Wed 5 Oct 2016
8_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename8_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14935932
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACATGCAATTTCTTTTTGGAATATAATCCCAGGTTCTCTGTTGCCCC553500.37058283339800957No Hit
CTGAGACTGTAACCTCAAAGCTTTCGCGTGAAGAAGTCAGATAACGTGCT468970.3139877712351663No Hit
CCCGCAGAGAAGGATACAAGCGAATACCGCCCCCCCTTGGGTAAAACCCG451870.30253887069116275No Hit
CCCTGAGACTGTAACCTCAAAGCTTTCGCGTGAAGAAGTCAGATAACGTG416550.2788912000938408No Hit
CCCCGAACTACCCTCCTTCATTCCTGGATGCCGTGAGTTGAGGGAAGGCA318790.21343830435221586No Hit
CCGCAGAGAAGGATACAAGCGAATACCGCCCCCCCTTGGGTAAAACCCGC285810.19135732540828385No Hit
CTCCAATTGTTACTCGATTTTGGATGGGTTTAAATATCCCCCATTGAAAA270150.18087254280482798No Hit
CGTAAATTTAATATATTCATTGGCGAGACGAGGAACGGGATGAAAAATAA244830.1639201356835315No Hit
CTCGCAATTTCCAGCTAAGTAGGGTAAAACCAACCTGTCTCACGACGGTC233640.15642813585385898No Hit
GTCAATTTCTTTAAGTTTCACTCTTGCGAACGTACTCCCCCCTGAGACTG222540.14899639339547074No Hit
GTACAAATGGCAGGGACGTAATCGGCACAGTTTGATGAGCTGCGCCTACG220490.14762386438288552No Hit
CTCTAATCATCTTCGATCCCCACACTTTGGTTCTTGATTGAGGAAGGTAT212630.1423613872907295No Hit
CTTCGCCAAATATGAGACCACACGAAGAAGAAGGAGCGCTTTGTCCCTCC204230.1367373659708681No Hit
CCTGAGACTGTAACCTCAAAGCTTTCGCGTGAAGAAGTCAGATAACGTGC203150.13601427751545736No Hit
GTTACGACTTTTGCTTCCTCTATTGAAGAAATATCGGTGAACTTTCGGTC203120.13599419172502927No Hit
CCATAATCTCCAATGGACTTTTAAAACCAACAAAAGCCGAAACGGTGGCC194540.1302496556625994No Hit
CTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATATCGGTGAACTTTCG190380.12746442605657285No Hit
CTCGATTTTGGATGGGTTTAAATATCCCCCATTGAAAACGACAATGCAAA188990.1265337844334053No Hit
CCACATGCAATTTCTTTTTGGAATATAATCCCAGGTTCTCTGTTGCCCCT187200.12533533227119675No Hit
ACGACTTTTGCTTCCTCTATTGAAGAAATATCGGTGAACTTTCGGTCAAG164330.1100232647015265No Hit
CCCCCCTGAGACTGTAACCTCAAAGCTTTCGCGTGAAGAAGTCAGATAAC149960.1004021710864779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGT49500.060.499302113
CTAGGTT50150.060.089497114
TAGGTTA53600.056.437275115
AGGTTAG56050.054.579433116
GCGCTAG55200.054.23316111
CGCTAGG56450.053.213097112
AGCTAAG59350.049.8526313
GGTTAGG61900.049.73788117
CTAAGTA63800.046.83120715
CCCTTAG65300.046.39911393
GGCGCTA64800.046.13904110
TAGGGTA65600.045.28725420
TTAGGAA69450.044.282288119
TAGGAAG71100.043.43512120
CCCACAT176300.042.8182641
TTAGCGT70650.042.3085496
CAGCTAA70700.042.0950212
TAAGTAG71700.041.91486716
AGTAGGG71450.041.57585518
GTAGGGT72750.040.6749219
Produced by FastQC (version 0.11.5)