FastQCFastQC Report
Wed 5 Oct 2016
8_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename8_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8309
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATGGG170.20459742447948007No Hit
CAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGTTA120.14442171139728005No Hit
CCGCAAATGACGGGGCGGCCAGATGCACGCGAGGCGGTGGCACAATACTG120.14442171139728005No Hit
GTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG100.12035142616440005No Hit
CTGAAGCCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGA90.10831628354796004No Hit
GTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG90.10831628354796004No Hit
CACTCAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTG90.10831628354796004No Hit
TCAGAATCTTCCGCGAAATCTCGTATTGTGCTTCCTGTCACACTCAATGA90.10831628354796004No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCCCA100.009568282130.27586115
TAACAGT100.00976824129.38356106
GTAACAG100.00976824129.38356105
AATCCCT100.009970954128.503499
GACAACA100.009970954128.503496
GGAATGA100.009970954128.5034102
ATCCCTT100.009970954128.5034100
TGGTGGA300.00488252956.8975911
Produced by FastQC (version 0.11.5)