FastQCFastQC Report
Tue 25 Oct 2016
1_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15654305
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGCCGTAAGCAGTTCTGAGTTGGCAATTTGTGGAAACCGGTCCCTCC235760.1506039392997645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGT8700.031.417295113
TAAGCAG133250.028.9223069
ACCGGTC135550.027.1648139
AGTTCTG138850.026.68307114
AACCGGT138800.026.65258438
CTAGGTT10700.026.390148114
CGTAAGC142500.026.3188087
CTGAGTT140100.026.28326818
TCTGACT140900.025.65734157
TCTGAGT145350.025.64936617
GTTCTGA144300.025.5567715
CTGACTT141450.025.40389858
GTAAGCA150100.025.2929658
CCGGTCC152250.024.2638440
AAACCGG154300.024.19825737
CCGTAAG150250.024.1199956
GAGTTGG155200.024.0236420
AGTTGGC164900.022.82265521
GAAACCG167550.022.38650136
TTCTGAG168150.022.13711216
Produced by FastQC (version 0.11.5)