FastQCFastQC Report
Tue 25 Oct 2016
1_R1U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1_R1U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6858
Sequences flagged as poor quality0
Sequence length3-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCTCCTCCTCCAAGAATTTGTGAAGAGTTTTGTGT160.23330417031204434No Hit
CCCGTGTTCGTCTGATGGAACGACGAAGCCGCGCACGCAATCCATGAACC100.1458151064450277No Hit
CTGCGCAGGATGTCCACTAATTCCGCCTGTGTCTGCAGGGCACCCAACTG80.11665208515602217No Hit
GCGTCTTTCCAGTCTCAGCAACTTCCCGTGTTCGTCTGATGGAACGACGA80.11665208515602217No Hit
CTTTCCAGTCTCAGCAACTTCCCGTGTTCGTCTGATGGAACGACGAAGCC70.1020705745115194No Hit
GGCTCCTCCTCCAAGAATTTGTGAAGAGTTTTGTGT70.1020705745115194No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATATTA100.0065628313147.29591119
CTCACCT100.009048157132.4311889
TCTCACC100.009048157132.4311888
Produced by FastQC (version 0.11.5)