FastQCFastQC Report
Tue 25 Oct 2016
1_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15654305
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTAATATAAAAGGCTTTGGAAGGGGATTTTGGGGGACACCCGCCAGAT166010.10604750578195583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT457800.054.7385331
ACAGTTT604300.041.6878172
TTGATAC203350.039.1388363
TGATACA205100.038.720714
ATACAGT217000.036.6246156
GATACAG220100.036.1620335
CAGTTTC720200.034.924143
ATTGATA230950.034.784072
AGTTTCT898000.028.0412244
GTTTCTG1267500.027.473091
TATTGAT315100.027.3543071
TTCTGTA1325650.026.1174343
TCTGTAC1330850.025.9764394
ACATAAG61000.025.3415436
TGTACTA1408100.024.5192416
CTGTACT1420050.024.3500295
TTTCTGT1440850.024.1522882
TACTATA1437600.024.0261678
GTACTAT1451750.023.775247
CATAAGG62100.023.6908027
Produced by FastQC (version 0.11.5)