FastQCFastQC Report
Tue 25 Oct 2016
1_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10900
Sequences flagged as poor quality0
Sequence length1-126
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG230.2110091743119266No Hit
CACTCAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTG170.15596330275229356No Hit
GCAGGACATACCAGTGGTGTGTGGATCAGAATCTTCTGCGAAATCTCGTA160.14678899082568808No Hit
CCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCT160.14678899082568808No Hit
CAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGTTA150.13761467889908258No Hit
CTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATGGG140.12844036697247707No Hit
CAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATC130.11926605504587157No Hit
CCGCAAATGACGGGGCGGCCAGATGCACGCGAGGCGGTGGCACAATACTG130.11926605504587157No Hit
CGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCTCCA130.11926605504587157No Hit
ATCAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGT130.11926605504587157No Hit
GGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG120.11009174311926606No Hit
TGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCTCCACTGCCTCTGAAG120.11009174311926606No Hit
GTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG120.11009174311926606No Hit
CTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACC120.11009174311926606No Hit
GAAGCCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGAAA120.11009174311926606No Hit
CTCTACTTGTCTAAGGGGCAGCAGGACATACCAGTGGTGTGTGGATCAGA120.11009174311926606No Hit
GTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG110.10091743119266054No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAATA100.00839458136.27684120
CACACAA100.0085379565135.51125115
ATAATTA100.0085379565135.51125118
CACCCCC100.0085379565135.51125118
ATGCAAT100.0085379565135.51125119
AATCGGA100.0085379565135.51125114
CATAATT100.0085379565135.51125117
CCAGAGC100.00882957134.00557111
AGCATAT100.00882957134.00557113
TTACTCA100.009279276131.80875103
ATTACTC100.009279276131.80875102
GGAGCAA100.00974394129.682893
CTGCATT100.009902193128.989390
GAGTGTA153.2617687E-4112.7149627
AAAGAGT153.323273E-4112.19070424
AAGAGTG200.001043000484.1430225
GATCGGA456.279333E-874.1044541
AGAGTGT250.00248201767.62897526
TACAGTT250.002623874266.694011
ATCGGAA501.04388655E-555.5783422
Produced by FastQC (version 0.11.5)