FastQCFastQC Report
Tue 25 Oct 2016
2_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename2_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14737426
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGCCGTAAGCAGTTCTGAGTTGGCAATTTGTGGAAACCGGTCCCTCC166200.11277410315749846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCAG109150.026.8460879
ACCGGTC105050.026.61455739
AACCGGT109300.025.84362438
CTGACTT110850.024.9860258
CTGAGTT112900.024.82922418
CGTAAGC116400.024.6743727
AGTTCTG115900.024.5345914
GCTAGGT10050.024.425837113
GTTCTGA116150.024.33310715
TCTGACT114800.023.81252157
GTAAGCA125600.023.328998
CCGGTCC120150.023.27211440
CTAGGTT10950.023.186916114
TCTGAGT124300.022.9243417
AAACCGG123600.022.57144737
GAGTTGG129750.021.9189520
CCGTAAG126650.021.5800636
CGAACTA39050.021.5000424
AGTTGGC136000.020.7019321
GAAACCG139200.020.33300836
Produced by FastQC (version 0.11.5)