FastQCFastQC Report
Tue 25 Oct 2016
2_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename2_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14737426
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTAATATAAAAGGCTTTGGAAGGGGATTTTGGGGGACACCCGCCAGAT155210.10531689862259529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT427800.056.4520531
ACAGTTT566450.042.8297962
TTGATAC189300.039.180893
TGATACA192000.038.6901324
GATACAG197700.037.3130075
ATACAGT200150.037.027846
CAGTTTC671800.036.1055643
ATTGATA211350.035.0375062
AGTTTCT839800.028.9861284
GTTTCTG1178550.027.6398471
TATTGAT294700.026.660751
ACATAAG58750.026.4326296
TTCTGTA1232450.026.2913423
TCTGTAC1235450.026.1711314
TGTACTA1294150.025.0333126
CTGTACT1309850.024.6727525
TACTATA1314350.024.6552498
GTACTAT1329900.024.357347
TTTCTGT1336900.024.3320142
ACTATAT1348200.024.050999
Produced by FastQC (version 0.11.5)