FastQCFastQC Report
Tue 25 Oct 2016
2_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename2_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9658
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG220.22779043280182232No Hit
CCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCT170.17601987989231727No Hit
GTTTCTGTACTATATTGTTATAAGGTGCTCTCGGCTAAAATATACTTGAA160.16566576931041624No Hit
CTGAAGCCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGA150.1553116587285152No Hit
GTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG150.1553116587285152No Hit
CTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATGGG140.14495754814661418No Hit
CACTCAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTG140.14495754814661418No Hit
ATCAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGT140.14495754814661418No Hit
CAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATC130.13460343756471319No Hit
GTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG120.12424932698281217No Hit
CAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGTTA120.12424932698281217No Hit
CTCAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAA120.12424932698281217No Hit
CGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCTCCA100.10354110581901013No Hit
GAAGCCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGAAA100.10354110581901013No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTTG100.009182299132.179106
ATGACTT100.009182299132.179105
ACTTGGA100.009182299132.179108
ATGGAAG100.009182299132.179107
CAATAAG100.009182299132.179101
AATAAGA100.009182299132.179102
ATATTAA100.009182299132.179109
CCAATAA100.009353812131.3681100
TCTAGTG100.009353812131.3681100
CATGAGT100.009881089128.9939787
GATCGGA200.001086735783.211141
ATCGGAA250.002634516766.568912
TTCATGG300.004581500357.87297455
TACAGTT350.00997905147.549221
ACAGTTT350.00997905147.549222
Produced by FastQC (version 0.11.5)