FastQCFastQC Report
Tue 25 Oct 2016
3_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename3_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16161612
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGT10900.026.441996113
CTAGGTT12800.023.20841114
TAAGGGG43600.019.87234371
GAATCTA22150.019.799433100
TAAGCAG82550.019.1246369
CGAACTA41950.019.0226884
CTAGGCT17150.018.959839111
CCCCGAT52100.018.319491
GACTAGG17950.018.262836109
TGGACTA29050.017.840431118
ACCGGTC82750.017.77161839
CGAATTA42750.017.72868119
TAGGTTA16900.017.696379115
GGACTAC28800.017.626762119
CGTAAGC87200.017.5737137
CTGAGTT84350.017.5541218
CCTATGG36900.017.39577567
TCCTATG39100.016.70466
GTTCTGA88100.016.60852815
GTAAGCA98300.016.5895188
Produced by FastQC (version 0.11.5)