FastQCFastQC Report
Tue 25 Oct 2016
3_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename3_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16161612
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTAATATAAAAGGCTTTGGAAGGGGATTTTGGGGGACACCCGCCAGAT176810.10940121567081303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT357850.057.290741
ACAGTTT501950.041.1210482
CAGTTTC599200.034.467383
ATACAGT158200.031.5610286
TGATACA160600.031.0874584
GATACAG160500.030.8185625
TTGATAC162400.030.6354543
GTTTCTG1128850.030.157961
TTCTGTA1192450.028.3567333
TCTGTAC1196850.028.1756734
ACATAAG65700.027.91526
AGTTTCT766650.026.947144
TGTACTA1255800.026.8132426
ATTGATA189200.026.509272
CTGTACT1274350.026.4123045
TACTATA1280050.026.299338
TTTCTGT1302350.026.1452242
GTACTAT1295900.025.9890527
CATAAGG66850.025.8782027
ACTATAT1320150.025.5060469
Produced by FastQC (version 0.11.5)