FastQCFastQC Report
Tue 25 Oct 2016
3_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename3_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10534
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG170.16138219100056958No Hit
GTTTCTGTACTATATTGTTATAAGGTGCTCTCGGCTAAAATATACTTGAA140.13290298082399848No Hit
ATCAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGT130.12340991076514145No Hit
GTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG120.1139168407062844No Hit
CTCTACTTGTCTAAGGGGCAGCAGGACATACCAGTGGTGTGTGGATCAGA120.1139168407062844No Hit
CAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATC110.10442377064742737No Hit
CCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCT110.10442377064742737No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCATG100.007920773138.90419116
AGATGAT100.007920773138.90419119
AGGCCAA100.008209475137.26035107
CCGGTGG100.008807677134.08672102
GAGTGTA153.2706995E-4112.60680427
GTGTAGG252.3271466E-588.7923516
GATCGGA200.001108627282.846431
AAGAGCG250.002636734966.594267
Produced by FastQC (version 0.11.5)