FastQCFastQC Report
Tue 25 Oct 2016
4_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename4_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15331136
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGT12450.027.351336113
CTAGGTT12850.026.675694114
TAAGGGG45650.021.57058571
GAATCTA23900.020.649563100
TAAGCAG82400.020.4711559
CGAACTA47150.019.4233264
ACCGGTC83550.019.31063739
CCTATGG42100.019.01873467
CTAGGCT17000.018.994152111
CCCCGAT48100.018.803481
GTTCTGA88350.018.50413115
TAGGTTA18300.018.480955115
AGTTCTG89150.018.142714
AACCGGT90450.018.09234438
GACTAGG17300.018.056414109
CTGAGTT89450.018.01851318
CGTAAGC90500.017.9336647
CGAATTA40850.017.884245119
TCCTATG45250.017.68343566
CTGACTT89850.017.54090758
Produced by FastQC (version 0.11.5)