FastQCFastQC Report
Tue 25 Oct 2016
4_R1U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename4_R1U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7073
Sequences flagged as poor quality0
Sequence length1-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCTCCTCCTCCAAGAATTTGTGAAGAGTTTTGTGT120.1696592676374947No Hit
GTGGTTTCTGGCGGAGGTAAACGGATTTGCGTGCCAGTCGTCTCTGCTTT90.12724445072812102No Hit
CTGCGCAGGATGTCCACTAATTCCGCCTGTGTCTGCAGGGCACCCAACTG90.12724445072812102No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGATG100.008687811134.31897102
AAAAGCC100.00891558133.1709495
GACGACG100.00891558133.1709499
TCGTGAG100.009622607129.8416689
CGTGAGT100.009622607129.8416690
Produced by FastQC (version 0.11.5)