FastQCFastQC Report
Tue 25 Oct 2016
4_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename4_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15331136
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTAATATAAAAGGCTTTGGAAGGGGATTTTGGGGGACACCCGCCAGAT180050.11744074281253522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT396450.054.337011
ACAGTTT533100.040.594862
TTGATAC186150.036.634433
TGATACA185850.036.538634
ATACAGT190700.035.3681566
GATACAG193200.035.1488045
CAGTTTC635400.034.1508673
ATTGATA214050.032.1839452
GTTTCTG1132550.029.6344241
ACATAAG64050.029.155486
TTCTGTA1186400.028.0511253
TCTGTAC1192750.027.8296744
AGTTTCT786600.027.5872084
TGTACTA1251100.026.5657336
CATAAGG64800.026.4027337
CTGTACT1260900.026.316495
TACTATA1265050.026.2247568
TTTCTGT1291850.025.9719452
GTACTAT1280950.025.95237
GATAAAC76100.025.6785551
Produced by FastQC (version 0.11.5)