FastQCFastQC Report
Tue 25 Oct 2016
4_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename4_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9466
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATGGG170.1795901119797169No Hit
CACTCAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTG170.1795901119797169No Hit
GCCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGA130.1373336150433129No Hit
CGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCTCCA130.1373336150433129No Hit
CCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCT130.1373336150433129No Hit
GTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG120.1267694908092119No Hit
ATCTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATG120.1267694908092119No Hit
CCGCAAATGACGGGGCGGCCAGATGCACGCGAGGCGGTGGCACAATACTG110.11620536657511094No Hit
CTGAAGCCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGA110.11620536657511094No Hit
CTCAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAA100.10564124234100994No Hit
GTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG100.10564124234100994No Hit
GCAGGACATACCAGTGGTGTGTGGATCAGAATCTTCCGCGAAATCTCGTA100.10564124234100994No Hit
TGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCTCCACTGCCTCTGAAG100.10564124234100994No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATATC100.007966947138.54120
AGACGCA100.008793057134.07097106
CAACATC100.009138804132.36305100
ATGGCCG100.009315012131.5253198
GATCGGA305.3002805E-791.626981
ATCGGAA305.3002805E-791.626982
TCGGAAG252.4259783E-587.961913
GGGAAAG300.00550032855.26861621
CGGAAGA300.00561795854.9761924
TCTGTAC550.001196189739.982683
TGTACTA550.001196189739.982685
TACTATA550.001196189739.982687
ACTATAT550.001196189739.982688
GTACTAT600.001834613636.6507956
GGAAGAG600.001834613636.6507955
CTGTACT600.001834613636.6507954
CTATATT700.003907365331.4149679
TATATTG700.003907365331.41496710
Produced by FastQC (version 0.11.5)