FastQCFastQC Report
Tue 25 Oct 2016
5_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename5_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16185108
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGTT12950.032.153557114
GCTAGGT12900.031.54768113
TAGGTTA16450.025.053766115
TAAGGGG49750.021.34020271
AGGTTAG20850.020.563696116
GCGCTAG20900.020.521498111
TGGACTA33450.020.100828118
GGACTAC33400.020.053743119
GAATCTA22200.019.920086100
CTAGGCT17400.019.642477111
CCCCGAT46800.019.3406091
GACTAGG18600.019.227852109
TAGCTAA18300.017.78924443
CGCTAGG23100.017.509026112
CGAACTA47500.017.3397924
AGACCTA20000.017.1388497
TAATCTA25400.016.932241119
AGCTAAG22650.016.9048813
ACTAGGC21050.016.77492110
ACGCCTA38400.016.77432368
Produced by FastQC (version 0.11.5)