FastQCFastQC Report
Tue 25 Oct 2016
5_R1U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename5_R1U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9635
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATCG100.009336234131.4606120
AGCACAA100.009336234131.4606109
ATGCAAG100.009336234131.4606113
CGTTTTA100.009336234131.4606108
ATCTTTT100.0098559875129.1130895
GATGCGC100.0098559875129.1130896
GCGCATC100.0098559875129.1130896
CAAAGCG250.001542567576.1087792
Produced by FastQC (version 0.11.5)