FastQCFastQC Report
Tue 25 Oct 2016
5_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename5_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16185108
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTAATATAAAAGGCTTTGGAAGGGGATTTTGGGGGACACCCGCCAGAT180280.11138634354494269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT347300.056.910641
ACAGTTT483300.041.0649222
CAGTTTC572300.034.760993
ATACAGT150950.032.3722466
TTGATAC153500.032.059933
GATACAG155700.031.9443555
TGATACA156000.031.8810524
GTTTCTG1094500.031.053681
TTCTGTA1169100.028.8303593
TCTGTAC1174250.028.629874
ATTGATA176000.028.2242742
TGTACTA1233550.027.282886
AGTTTCT736350.027.0876734
TACTATA1254150.026.8746748
CTGTACT1253100.026.8206795
TTTCTGT1274150.026.6893942
GTACTAT1267850.026.5693077
ACTATAT1292200.026.0623159
ACATAAG66450.025.4151046
CTATATT1334750.025.28049310
Produced by FastQC (version 0.11.5)