FastQCFastQC Report
Tue 25 Oct 2016
5_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename5_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10660
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCTCCA150.14071294559099437No Hit
GTGCTCTTCCGATC140.13133208255159476Illumina Multiplexing Adapter 1 (100% over 14bp)
GTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG130.12195121951219512No Hit
GCTCTTCCGATC130.12195121951219512Illumina Single End Adapter 1 (100% over 12bp)
CTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATGGG120.1125703564727955No Hit
CAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATC110.10318949343339587No Hit
GTTTGAATCAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAA110.10318949343339587No Hit
CTCTACTTGTCTAAGGGGCAGCAGGACATACCAGTGGTGTGTGGATCAGA110.10318949343339587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTGT100.009678191129.9615598
GGTTGTC100.009678191129.9615599
CGCAGCA100.00983887129.2513792
Produced by FastQC (version 0.11.5)