FastQCFastQC Report
Tue 25 Oct 2016
6_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename6_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14124084
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGTT12050.035.074512114
GCTAGGT11950.034.018093113
TAGGTTA15950.026.228252115
TAAGGGG52000.024.9231371
AGCTAAG18450.023.90429713
GACTAGG16350.022.680742109
GCGCTAG20200.020.885027111
GTAAGGG62650.020.7679670
GAATCTA20300.020.539942100
AGGTTAG20750.020.286741116
AGTAAGG64950.020.11348369
TGGACTA33800.020.058388118
AGACCTA19750.019.88270297
GGACTAC34500.019.777866119
CTAGGCT17300.019.353954111
TGACTAG19400.019.346758108
ACGCCTA36350.019.32876468
CTAATCA51750.019.2859023
GCCGAAC66450.019.14426462
CCCCGAT40800.018.92121
Produced by FastQC (version 0.11.5)