FastQCFastQC Report
Tue 25 Oct 2016
6_R1U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename6_R1U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11058
Sequences flagged as poor quality0
Sequence length2-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTTCCGATC130.11756194610236934Illumina Single End Adapter 1 (100% over 12bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCTC100.008243034137.12708109
GCTTCCA100.008243034137.12708110
TTAAAAG100.008660532134.89131101
CCCCACC209.485552E-486.1805655
TCACAAA350.009546963548.1318713
Produced by FastQC (version 0.11.5)