FastQCFastQC Report
Tue 25 Oct 2016
6_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename6_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14124084
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTAATATAAAAGGCTTTGGAAGGGGATTTTGGGGGACACCCGCCAGAT164880.1167367738679549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT321400.055.5477521
ACAGTTT440800.040.67372
TTGATAC146200.036.2586943
ATACAGT145250.036.095476
TGATACA145050.036.0658234
GATACAG149050.035.255255
CAGTTTC523850.034.436823
ATTGATA167200.031.7038862
GTTTCTG953000.029.5532721
ACATAAG63600.027.8434986
TTCTGTA1015700.027.5359423
AGTTTCT656600.027.5275154
TCTGTAC1016350.027.4612064
CATAAGG64850.026.592267
TGTACTA1064600.026.23256
TACTATA1076950.025.9400068
CTGTACT1077700.025.8828645
GTACTAT1090200.025.5861877
GATAAAC72700.025.4755481
TTTCTGT1112150.025.3247932
Produced by FastQC (version 0.11.5)