FastQCFastQC Report
Tue 25 Oct 2016
6_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename6_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12468
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACTC100.009732373129.83409117
GAGATGA205.83873E-497.37557117
CACGACT209.1816636E-486.9567946
TTTTGCA250.001756848773.8183473
GAGGGCA250.002414957968.162920
TTTGCAT300.003619830461.51528574
ACAAACG350.005377745355.64319117
ACTATAT551.6668562E-551.431189
CTATATT453.9126538E-450.28826510
TATATTG453.9126538E-450.28826511
GGAGGGC350.00914983348.68778619
AGTTTCT350.00929737348.4922561
GTTTCTG550.001051876241.1449431
Produced by FastQC (version 0.11.5)