FastQCFastQC Report
Tue 25 Oct 2016
7_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename7_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14122032
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGTT9550.030.53747114
GCTAGGT10050.028.81382113
TAAGGGG43400.022.77725871
CGAACTA49750.022.6386244
TAAGCAG93900.022.6370569
TAGGTTA13150.021.797102115
ACCGGTC91300.021.47415439
CTAGGCT12450.021.309072111
AACCGGT95900.020.68343438
CGTAAGC99900.020.640737
GACCGAA58000.020.52602680
CTGAGTT97050.020.35962518
AGTTCTG99850.020.24929814
GTTCTGA97750.020.21203615
CTGACTT95850.020.09276658
GAACTAC57750.020.0028865
CCTATGG44050.019.85261267
GTAAGCA106650.019.6595828
TCTGACT98850.019.41808157
GCGCTAG15900.019.25239111
Produced by FastQC (version 0.11.5)