FastQCFastQC Report
Tue 25 Oct 2016
7_R1U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename7_R1U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6391
Sequences flagged as poor quality0
Sequence length2-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTTCCGATC100.1564700359881083Illumina Single End Adapter 1 (100% over 12bp)
CTGCGCAGGATGTCCACTAATTCCGCCTGTGTCTGCAGGGCACCCAACTG90.14082303238929744No Hit
GTCGGCTCCTCCTCCAAGAATTTGTGAAGAGTTTTGTGT80.12517602879048662No Hit
GTGGTTTCTGGCGGAGGTAAACGGATTTGCGTGCCAGTCGTCTCTGCTTT70.10952902519167579No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTTC200.001023168784.141736
Produced by FastQC (version 0.11.5)