FastQCFastQC Report
Tue 25 Oct 2016
7_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename7_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14122032
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTAATATAAAAGGCTTTGGAAGGGGATTTTGGGGGACACCCGCCAGAT158630.11232802758129991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT353150.054.5006371
ACAGTTT481350.040.2984922
TTGATAC163400.038.6860583
TGATACA163650.038.5921974
GATACAG168150.037.5600665
ATACAGT170450.036.6821566
ATTGATA188300.033.908642
CAGTTTC587300.032.9688383
GTTTCTG1033050.028.8276461
TTCTGTA1100650.026.8735543
AGTTTCT726650.026.6785744
TCTGTAC1107100.026.6651734
ACATAAG53950.026.242146
TATTGAT266200.025.8517441
TGTACTA1155350.025.5783066
TACTATA1170650.025.231258
CTGTACT1174200.025.1418295
GTACTAT1186400.024.8908067
TTTCTGT1194550.024.8873982
ACTATAT1206350.024.4659299
Produced by FastQC (version 0.11.5)