FastQCFastQC Report
Tue 25 Oct 2016
7_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename7_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10364
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG160.1543805480509456No Hit
CTCTACTTGTCTAAGGGGCAGCAGGACATACCAGTGGTGTGTGGATCAGA150.14473176379776148No Hit
CTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATGGG140.1350829795445774No Hit
CAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGTTA130.1254341952913933No Hit
GCCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGA110.10613662678502508No Hit
GCAGGACATACCAGTGGTGTGTGGATCAGAATCTTCTGCGAAATCTCGTA110.10613662678502508No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAAT100.009980661128.6101782
TGGCGGT153.324028E-4112.1379339
GATCGGA252.4350787E-587.976811
GGAAAGA200.001127948682.4782622
CCGCAAC250.002289396768.9818248
TCGGAAG250.002734227665.9826053
ATCGGAA351.2904374E-462.8405842
GAAAGAG300.005521829255.25242623
TTTCTGT300.005629448754.9855086
Produced by FastQC (version 0.11.5)