FastQCFastQC Report
Tue 25 Oct 2016
8_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename8_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14066657
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGT8900.032.948994113
CTAGGTT10450.027.581402114
CTAGGCT13600.024.272621111
GACTAGG13650.023.908623109
TAAGCAG81700.021.8200429
TAAGGGG38350.020.94206471
CCCCGAT43000.020.7336831
ACCGGTC81750.020.34555839
TAGGTTA14350.020.214869115
TGACTAG17050.020.19268108
AACCGGT84700.019.84044538
ACTAGGC16950.019.762415110
CGAATTA38100.019.513493119
CTGAGTT87200.019.25380318
CGAACTA40050.019.21954
CTGACTT89200.018.75842758
CGTAAGC92300.018.6235127
GTTCTGA91800.018.53943315
GTAAGCA97250.018.3899428
AGGTTAG16500.018.109203116
Produced by FastQC (version 0.11.5)