FastQCFastQC Report
Tue 25 Oct 2016
8_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename8_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14066657
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTAATATAAAAGGCTTTGGAAGGGGATTTTGGGGGACACCCGCCAGAT158110.11240055117573422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT337350.054.1479341
ACAGTTT459950.040.0052832
TTGATAC156600.034.5695653
TGATACA160550.033.720134
GATACAG163600.033.340895
ATACAGT163050.033.3130766
CAGTTTC555950.033.1285173
ATTGATA179900.030.3818972
GTTTCTG1046850.030.269431
TTCTGTA1115800.028.140213
TCTGTAC1120050.027.9981424
ACATAAG55850.027.5798026
CATAAGG53150.026.584887
TGTACTA1195900.026.2299026
TTTCTGT1209150.026.1640262
AGTTTCT709700.026.0747364
CTGTACT1208400.025.928445
TACTATA1215600.025.7453658
GTACTAT1234000.025.411577
ACTATAT1249300.025.0382219
Produced by FastQC (version 0.11.5)