FastQCFastQC Report
Tue 25 Oct 2016
8_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename8_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32792
Sequences flagged as poor quality0
Sequence length1-126
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT152.7791943E-4117.8908136
TAGATCT205.923048E-6117.7071632
TCGGGTA152.974366E-4115.9018514
AGACGGG152.974366E-4115.9018518
CGGAATA251.4073521E-7115.54744
GATCGGA1700.0101.953591
GATCGGT358.94579E-999.040631
GATCGGG650.097.770881
GTAGATC303.9479164E-797.18107631
GGGTCGT304.0958912E-796.5848711
TCGGAAG1150.095.45223
TGTAGGG800.094.1702517
TGCAGGG251.9385412E-592.7214717
CTCGGAA251.9683323E-592.437932
ATCGGAA1450.091.6410452
CGTGTAG900.090.14587415
GTAGGGA850.088.6308218
ATCACGG208.778978E-488.2803735
CGGGTAG209.3362614E-486.92638415
TCTTGTA209.3362614E-486.92638414
Produced by FastQC (version 0.11.5)