FastQCFastQC Report
Fri 4 Nov 2016
1_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15850241
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGT15000.032.232716113
CTAGGTT15400.031.623848114
GCGCTAG22700.022.519943111
TAGGTTA21600.022.376572115
CCCCGAT45450.020.841121
CGCTAGG25150.020.18839112
AGGTTAG24950.019.789595116
AGCTAAG25200.019.70703513
TAAGGGG43650.018.48077271
CTAAGTA27600.017.78482815
GAATCTA19350.017.447807100
GGTTAGG30300.017.12247117
GCTAAGT29450.016.86327714
CCTATGG34400.016.77610667
GGCCTAT22400.016.7600941
CTAACGG25600.016.03649732
TAGGGTA30800.015.93936820
CGAATTA41700.015.55182119
TCCTATG37750.015.43190466
CGAACTA27600.015.0620864
Produced by FastQC (version 0.11.5)