FastQCFastQC Report
Fri 4 Nov 2016
1_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15850241
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT297700.063.4236071
ACAGTTT438750.043.601162
CAGTTTC521300.036.553463
GTTTCTG1074700.034.4280051
TTCTGTA1133700.032.4388773
TCTGTAC1145000.032.1387254
TGTACTA1215050.030.2520036
CTGTACT1227200.029.9594695
TTTCTGT1241850.029.7899882
TACTATA1249250.029.4586058
GTACTAT1263250.029.1260837
ACTATAT1293450.028.4271079
AGTTTCT681800.028.0331134
ACATAAG68100.027.4885946
CTATATT1341250.027.47562810
TATATTG1422250.025.915811
GATAAAC76150.025.7203921
CATAAGG71950.025.69737
TGATACA110900.023.444654
GATACAG111200.023.0195
Produced by FastQC (version 0.11.5)