FastQCFastQC Report
Fri 4 Nov 2016
1_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12502
Sequences flagged as poor quality0
Sequence length1-126
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCT170.13597824348104304No Hit
CGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCTCCA160.1279795232762758No Hit
CTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATGGG150.11998080307150856No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACG100.009112073132.70335120
CCAGCGC100.009243633132.07144111
ATTAGAC100.009243633132.07144115
TCTCTTC100.009243633132.07144116
CACCTCT100.009376448131.4455103
TTCTACG100.009510524130.8254998
CAGACGG100.009645867130.2112794
GAAAGAG153.3238425E-4112.28744523
ATCGGAA550.0100.854542
GATCGGA500.099.8461
CGTGTAG351.0652002E-895.8582915
AAAGAGT252.2128903E-589.8299624
TCGGAAG501.4551915E-1188.7523
AGCTTCG200.001077330883.53915410
CGGAAGT200.001094722283.2054
GTGTAGG351.2218734E-679.8819116
TGTAGGG305.578001E-574.5564517
TCGTGTA250.002611702566.8313214
AGTTTCT250.002653787166.5639951
AAGAGTG351.1729814E-464.1642625
Produced by FastQC (version 0.11.5)