FastQCFastQC Report
Fri 4 Nov 2016
2_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename2_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17229959
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGT15000.028.23727113
CTAGGTT15050.027.8827114
CCCCGAT48850.020.9072041
TAGGTTA21000.020.459162115
GAATCTA21300.019.180672100
TAAGGGG44350.018.56397271
CGAATTA50050.018.132113119
CCTATGG37700.017.91490267
AGCTAAG25450.017.67962513
AGGTTAG23850.017.258026116
CGCTAGG26150.016.868559112
TCCTATG40500.016.66564866
GCGCTAG26350.016.622559111
CATCTAA113000.016.4364135
CCCGATT60850.015.451812
TGTACTA22300.015.2610036
TAGCTAA15600.015.19006343
TCTAACC58350.015.1249547
GGCCTAT22950.015.0853631
CTAGGCT12250.015.084335111
Produced by FastQC (version 0.11.5)