FastQCFastQC Report
Fri 4 Nov 2016
2_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename2_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17229959
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT330400.063.3280031
ACAGTTT466550.045.2678152
CAGTTTC570400.036.966373
GTTTCTG1168250.034.3529661
TTCTGTA1237450.032.2646373
TCTGTAC1246900.032.1218574
TGTACTA1312350.030.4840586
CTGTACT1325750.030.18245
TACTATA1341950.029.8224268
GTACTAT1361000.029.4167827
TTTCTGT1365850.029.3659882
ACTATAT1388100.028.844579
AGTTTCT748650.028.2418734
CTATATT1449200.027.75402810
ACATAAG73900.026.7902186
TATATTG1535300.026.21345311
TGATACA120050.025.3867534
TTGATAC122300.025.250153
CATAAGG75300.025.1433477
ATACAGT125050.023.81736
Produced by FastQC (version 0.11.5)