FastQCFastQC Report
Fri 4 Nov 2016
2_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename2_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11291
Sequences flagged as poor quality0
Sequence length1-126
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG250.22141528651138076No Hit
CTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATGGG210.18598884066955984No Hit
CTGAAGCCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGA210.18598884066955984No Hit
CCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCT170.15056239482773892No Hit
CAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATC150.13284917190682846No Hit
CTCAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAA150.13284917190682846No Hit
GTTTGAATCAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAA150.13284917190682846No Hit
CACTCAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTG120.10627933752546276No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAACA100.008259347137.02792120
CATCATC100.008682449134.76923102
ATGGAAA100.008682449134.76923109
TAATAGT100.008682449134.76923100
AATACAA100.008682449134.76923114
AGATTGT100.008682449134.76923116
TGCGTGC100.008972366133.3043494
GTAGGGA153.4092035E-4111.4909118
TCGGAAG153.5350618E-4110.486483
ATCGGAA153.5350618E-4110.486482
TAGGGAA250.002593791366.8945519
AGTTTCT250.00268919766.291891
TATCGTC350.00982511247.781829
ATATTGT350.00982511247.7818212
Produced by FastQC (version 0.11.5)