FastQCFastQC Report
Fri 4 Nov 2016
3_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename3_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15110673
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACATGCAATTTCTTTTTGGAATATAATCCCAGGTTCTCTGTTGCCCC259490.17172630232948594No Hit
CCCGCAGAGAAGGATACAAGCGAATACCGCCCCCCCTTGGGTAAAACCCG239000.15816635036705512No Hit
CCCCGAACTACCCTCCTTCATTCCTGGATGCCGTGAGTTGAGGGAAGGCA179500.11879020874847863No Hit
ACCGCACGCTCCACGTCTTGTGGTGCCATTCCGTCAATTTCTTTAAGTTT164070.10857888328335873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGT25750.048.22742113
CTAGGTT26800.046.914253114
TAGGTTA31150.040.424564115
GCGCTAG32950.037.403427111
AGGTTAG34350.037.124588116
CGCTAGG34250.036.415855112
GGTTAGG37550.034.383335117
AGCTAAG35800.033.30204813
CTAAGTA38250.031.77588315
TAAGGGG102300.031.35733271
CCCTTAG40500.030.37894893
GGCGCTA41600.029.762087110
TAGGGTA42000.029.22054720
ACGCCTA57550.028.91497268
AGTAAGG113750.028.25314569
GTAAGGG114650.028.054870
AGCCCGA59200.027.660759
TGTTACG52000.027.2649633
TCCCAGG120050.027.02240430
GTTACGA68850.026.9860671
Produced by FastQC (version 0.11.5)