FastQCFastQC Report
Fri 4 Nov 2016
3_R1U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename3_R1U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5985
Sequences flagged as poor quality0
Sequence length2-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTTCCGATC130.2172096908939014Illumina Single End Adapter 1 (100% over 12bp)
GTCGGCTCCTCCTCCAAGAATTTGTGAAGAGTTTTGTGT120.20050125313283207No Hit
CTGGGAGGTAGGGAGTGCCCCCTGTTGCTTCATCACCATCGCCAGTGGGAACTGCGCCCCCGCAGAATCCACA70.11695906432748539No Hit
CTCGTACACCTCATACGCATTCAGCTGACAAATAAAGGAAAACGTGAAAATGGAAAGGCC70.11695906432748539No Hit
CTGCGCAGGATGTCCACTAATTCCGCCTGTGTCTGCAGGGCACCCAACTG70.11695906432748539No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAA100.007240949142.41759116
AAGCACA100.0074841604140.86957115
CGCGAAG100.007732738139.35483111
TTCGCGA100.007732738139.35483109
GCGAAGC100.007732738139.35483112
AAGTTCG100.007732738139.35483106
GTTCGCG100.007732738139.35483108
GAAGTTC100.007986739137.87233105
TGCCGAA100.0082462225136.42105101
CGTGCCG100.008511245135.099
GCTTTTC100.008511245135.099
GTGCCGA100.008511245135.0100
GCGTGCC100.008781865133.6082598
CGCGTGC100.008781865133.6082597
CAACACC100.009627882129.5999988
GCTCCAG100.009627882129.5999990
CAGCGCG100.009627882129.5999994
TTCTCGC100.009921466128.3168285
ATACTTT200.00103553183.793138
Produced by FastQC (version 0.11.5)