FastQCFastQC Report
Fri 4 Nov 2016
3_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename3_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15110673
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTACCAGATCCGGACAGGGTGAGGATTGACAGATTGAGTGTTCTTTCT343810.22752792016609716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT286800.066.0445251
ACAGTTT414300.046.0689282
TTACCAG107950.039.1473923
CAGTTTC492400.038.538393
GTTTCTG991400.034.025051
TTCTGTA1045450.032.1385883
TCTGTAC1045200.032.1190074
CCAGATC134450.031.0028536
TACCAGA134900.030.89794
TGTACTA1085850.030.8861436
TCCGGAC136900.030.66524711
CTGTACT1096150.030.6052685
TACTATA1105150.030.3177348
CAGATCC138750.030.0427387
GTACTAT1119950.029.951597
ACCAGAT141200.029.9295985
AGTTTCT637350.029.8649794
CTTTACC147700.029.4348681
TTTCTGT1154200.029.2961312
ACTATAT1145000.029.2893099
Produced by FastQC (version 0.11.5)