FastQCFastQC Report
Fri 4 Nov 2016
3_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename3_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8662
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG220.2539829138767028No Hit
CCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCT190.21934888016624335No Hit
CGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCTCCA160.1847148464557839No Hit
ACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGAC130.15008081274532442No Hit
CAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATC120.1385361348418379No Hit
CTGAAGCCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGA120.1385361348418379No Hit
GCAGGACATACCAGTGGTGTGTGGATCAGAATCTTCCGCGAAATCTCGTA120.1385361348418379No Hit
GTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG110.1269914569383514No Hit
CTCAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAA110.1269914569383514No Hit
GTTTGAATCAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAA110.1269914569383514No Hit
CAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGTTA100.11544677903486493No Hit
CTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATGGG100.11544677903486493No Hit
CACTCAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTG100.11544677903486493No Hit
GCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGA90.10390210113137843No Hit
TGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCTCCACTGCCTCTGAAG90.10390210113137843No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCA100.008865189133.63889117
CGAGTCC100.008865189133.63889116
CAGCGTG100.009240954131.80821107
TGTTGTC100.009824202129.1543696
TGTAGGG251.5439946E-7112.5380117
CGTGTAG153.2592038E-4112.5380115
GGGAAAG207.0947317E-6112.5380121
GATCGGA207.5220178E-6111.2371
GTGTAGG304.5766137E-793.7816816
GATCGGC200.001071737383.427751
TCGGAAG200.001071737383.427753
GGAAGAG250.002538700167.130235
ATCGGAA250.002598248266.7421952
ATCGGCA300.005350060355.6184962
Produced by FastQC (version 0.11.5)