FastQCFastQC Report
Fri 4 Nov 2016
4_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename4_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14542741
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACATGCAATTTCTTTTTGGAATATAATCCCAGGTTCTCTGTTGCCCC146780.10093007913707602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGT23500.041.88649113
CTAGGTT24000.041.03188114
TAGGTTA28100.034.81225115
AGGTTAG30100.032.979633116
GCGCTAG30600.031.930927111
CGCTAGG33050.030.408241112
GGTTAGG35050.029.565985117
AGCTAAG33400.029.48873313
TAAGGGG68750.028.35185471
CTAAGTA37650.026.00754515
GGCGCTA39550.025.4035110
TAGGGTA38850.025.20777720
CCCTTAG40900.024.4796793
AGTAAGG81200.023.67104569
GTAAGGG82950.023.54526170
GCTAAGT43400.022.82688714
CCTATGG60100.022.73573167
TTAGCGT43500.022.62449596
TTAGGAA45450.022.349966119
TAAGTAG44150.022.30965216
Produced by FastQC (version 0.11.5)