FastQCFastQC Report
Fri 4 Nov 2016
4_R1U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename4_R1U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6175
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCTCCTCCTCCAAGAATTTGTGAAGAGTTTTGTGT110.17813765182186234No Hit
GCTCTTCCGATC110.17813765182186234Illumina Single End Adapter 1 (100% over 12bp)
GTGGTTTCTGGCGGAGGTAAACGGATTTGCGTGCCAGTCGTCTCTGCTTT110.17813765182186234No Hit
CTGGGAGGTAGGGAGTGCCCCCTGTTGCTTCATCACCATCGCCAGTGGGAACTGCGCCCCCGCAGAATCCACA80.12955465587044535No Hit
CTCGTACACCTCATACGCATTCAGCTGACAAATAAAGGCAAATGTGAAAACAGAAAGGCC70.11336032388663966No Hit
GGCTCCTCCTCCAAGAATTTGTGAAGAGTTTTGTGT70.11336032388663966No Hit
CCGCAACTTTCCACAGCGTCTTTCCAGTCTCAGCAACTTCCCGTGTTCGT70.11336032388663966No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAC100.0073353057141.8022117
TATCACG100.0073353057141.8022118
GGTATCA100.0073353057141.8022116
GCGACTA100.0075816824140.26086108
GGCGACT100.0075816824140.26086107
CGACTAC100.0075816824140.26086109
ACTACGG100.0075816824140.26086111
GACTACG100.0075816824140.26086110
GCGGTGC100.007833495138.75269102
ATGGCGA100.007833495138.75269105
ACATATC100.007833495138.7526997
TATCCAT100.007833495138.75269100
TGGCGAC100.007833495138.75269106
ATATCCA100.007833495138.7526999
CATATCC100.007833495138.7526998
CGCAACG100.007833495138.75269101
CAACGCT100.007833495138.75269103
CATCCCA100.0080908025137.276691
CCACATA100.0080908025137.276695
CACGTTC100.008353662135.8315785
Produced by FastQC (version 0.11.5)