FastQCFastQC Report
Fri 4 Nov 2016
4_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename4_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14542741
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTACCAGATCCGGACAGGGTGAGGATTGACAGATTGAGTGTTCTTTCT161360.11095569947921097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT264500.058.5441931
ACAGTTT400550.039.2206342
CAGTTTC451300.034.670113
GTTTCTG899150.032.162651
ACATAAG69600.030.3806366
TTCTGTA961200.029.787453
TCTGTAC964900.029.6683794
CATAAGG71550.028.6677637
TGTACTA999250.028.5180596
CTGTACT1012250.028.224255
TACTATA1017150.028.0240758
GATAAAC74900.027.6885971
GTACTAT1029400.027.6668627
ATACAGT109100.027.6195746
TTTCTGT1060300.027.2095782
ACTATAT1055400.027.0365569
GATACAG110850.026.9741485
AGTTTCT581150.026.9344774
TTGATAC114100.026.3557623
TGATACA114800.026.0454964
Produced by FastQC (version 0.11.5)