FastQCFastQC Report
Fri 4 Nov 2016
4_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename4_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11991
Sequences flagged as poor quality0
Sequence length1-126
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG220.18347093653573512No Hit
CCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCT170.14177299641397714No Hit
GCCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGA130.10841464431657077No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCATCA100.009459341131.03448119
ATATATG100.009459341131.03448113
ATTTAAG100.009459341131.03448109
TTCAGTA100.009885423129.126298
GGGTAAG153.270631E-4112.7118621
GGTAAGA153.270631E-4112.7118622
AGGGTAA153.3264793E-4112.2362920
GATCGGA401.8189894E-12111.297071
TGTAGGG252.2141388E-589.7890317
AAGAGTG209.92164E-485.2564125
ATCGGAA553.274181E-1180.943332
AAAAGTG250.002405597168.20512425
AAAGTGT250.002405597168.20512426
GAGAAAA250.002488698867.6271222
CGTCGTG250.002531043367.34177412
GTAGGGA250.002531043367.34177418
TCGGAAG501.3030512E-766.778243
AGAGTGT300.004953908756.8376126
CCGTCGT300.005211774356.11814511
GTGTAGG402.2716743E-456.1181416
Produced by FastQC (version 0.11.5)