FastQCFastQC Report
Fri 4 Nov 2016
5_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename5_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15968413
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACATGCAATTTCTTTTTGGAATATAATCCCAGGTTCTCTGTTGCCCC197590.12373803207619943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGT25400.052.21812113
CTAGGTT27100.049.5245114
TAGGTTA31700.041.95997115
GCGCTAG32500.040.909927111
AGGTTAG35800.037.60489116
CGCTAGG36000.037.352783112
AGCTAAG37350.034.06548713
GGTTAGG40800.033.751183117
GGCGCTA44200.031.113266110
CTAAGTA41700.030.92951415
TAAGGGG87150.029.52965271
TAGGGTA44200.029.1835820
CCCTTAG46750.028.43518393
GCTAAGT46200.027.9164614
TTAGGAA52600.026.271221119
TAAGTAG49250.026.0712516
TTAGCGT50000.026.06075196
GTAAGGG100950.025.53725870
TAGGAAG54700.025.436136120
AGTAAGG102500.025.25283669
Produced by FastQC (version 0.11.5)