FastQCFastQC Report
Fri 4 Nov 2016
5_R1U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename5_R1U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6183
Sequences flagged as poor quality0
Sequence length1-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCTCCTCCTCCAAGAATTTGTGAAGAGTTTTGTGT130.2102539220443151No Hit
GTGGTTTCTGGCGGAGGTAAACGGATTTGCGTGCCAGTCGTCTCTGCTTT100.16173378618793466No Hit
CTCGTACACCTCATACGCATTCAGCTGACAAATAAAGGCAAATGTGAAAACAGAAAGGCC90.1455604075691412No Hit
CCGCAACTTTCCACAGCGTCTTTCCAGTCTCAGCAACTTCCCGTGTTCGT90.1455604075691412No Hit
CTCACAACCATCCATTTGTCCATTACTTCGGCGTAGGCCCCATTTACGAG70.11321365033155426No Hit
CTCGTACACCTCATACGCATTCAGCTGACAAATAAAGGAAAACGTGAAAATGGAAAGGCC70.11321365033155426No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTCA100.008372447135.78787120
AGGCTTG100.008372447135.78787120
AAGGCTT100.008372447135.78787119
CGACAAA100.008630427134.43114
ACAAAGG100.008630427134.43116
GACAAAG100.008630427134.43115
TCGACAA100.008893631133.099113
ACGGCCT100.008893631133.099107
CGCTCAC100.009162114131.79411102
CACTGGC100.009162114131.79411101
CTCACGG100.009162114131.79411104
GCTCACG100.009162114131.79411103
TTCAAAC100.009435925130.5145694
CCTTGCG100.009435925130.5145697
GCACCCG100.009435925130.5145698
Produced by FastQC (version 0.11.5)