FastQCFastQC Report
Fri 4 Nov 2016
5_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename5_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15968413
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTAATATAAAAGGCTTTGGAAGGGGATTTTGGGGGACACCCGCCAGAT186590.1168494326893975No Hit
CTTTACCAGATCCGGACAGGGTGAGGATTGACAGATTGAGTGTTCTTTCT176740.1106810050566703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT300000.062.7627681
ACAGTTT441000.043.05072
CAGTTTC515000.036.8091743
GTTTCTG1053200.034.5522651
TTCTGTA1113150.032.5156943
TCTGTAC1122050.032.3095554
GATAAAC83500.030.8375131
TGTACTA1184600.030.5558266
ACATAAG84950.030.3134296
CTGTACT1195000.030.3055
TACTATA1203900.030.0839798
GTACTAT1219750.029.6959387
TTTCTGT1226800.029.6727982
ACTATAT1252000.028.9298299
AGTTTCT665350.028.621994
CATAAGG86300.028.0303447
CTATATT1303300.027.84683610
TTACCAG77900.027.558523
TATATTG1388400.026.13640811
TTGATAC111200.026.0706793
Produced by FastQC (version 0.11.5)