FastQCFastQC Report
Fri 4 Nov 2016
5_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename5_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9270
Sequences flagged as poor quality0
Sequence length1-126
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCT280.30204962243797195No Hit
CTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATGGG220.23732470334412084No Hit
GCCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGA150.16181229773462785No Hit
CAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGTTA150.16181229773462785No Hit
GTTTCTGTACTATATTGTTATAAGGTGCTCTCGGCTAAAATATACTTGAA150.16181229773462785No Hit
GTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG150.16181229773462785No Hit
CGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCTCCA140.15102481121898598No Hit
CTGAAGCCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGA130.14023732470334413No Hit
CTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACC130.14023732470334413No Hit
GTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG120.12944983818770225No Hit
CTCAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAA100.10787486515641855No Hit
CAACTCCCTGCGCCATATCTCAATTATTGCTGTAATGTTCATCGTCTTCT100.10787486515641855No Hit
GTTTGAATCAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAA100.10787486515641855No Hit
CCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGAC100.10787486515641855No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGTT100.008271305136.81334111
TCATCAT100.008271305136.81334119
AATGCAG100.008607621135.01315100
TGTTTTC204.6832068E-4102.61114
GTTTTCA204.6832068E-4102.61115
TTTTGAG200.001086002883.19729611
CCGCAAA250.002632753566.557841
GCAAATG250.002632753566.557843
CAAATGA250.002632753566.557844
CGCAAAT250.002632753566.557842
GACACAG300.003269210562.9509285
CGGGGCG300.00542094755.46486711
ACGGGGC300.00542094755.46486710
GGGGCGG300.00542094755.46486712
TGACGGG300.00542094755.4648678
GGCGGCC402.379047E-455.46486314
GCGGCCA454.255943E-449.302115
CAATACT350.00914088548.5919543
AATACTG350.00914088548.5919544
ACAATAC350.00914088548.5919542
Produced by FastQC (version 0.11.5)