FastQCFastQC Report
Fri 4 Nov 2016
6_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename6_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15346013
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACATGCAATTTCTTTTTGGAATATAATCCCAGGTTCTCTGTTGCCCC238450.15538237847185454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGT28850.048.8475113
CTAGGTT30650.046.31168114
TAGGTTA33900.042.19013115
AGGTTAG36700.039.648834116
CGCTAGG37600.038.30041112
GCGCTAG36850.038.07142111
GGTTAGG40550.035.992218117
AGCTAAG38550.034.7822713
TAAGGGG100500.032.97101671
GGCGCTA44250.031.634054110
CTAAGTA43250.031.00418315
TAGGGTA44200.030.60272620
CCCTTAG45400.029.79992593
GTAAGGG113800.029.20042270
AGTAAGG113600.029.08170769
TTAGGAA52450.028.254671119
TAGGAAG53550.028.161255120
CCCAGGT122550.027.38270831
CAGCTAA49200.027.37047412
CCAGGTT122850.027.36445432
Produced by FastQC (version 0.11.5)